Research Fellows Directory
Dr xiaoqun wang
University of Oxford
Molecular and cellular mechanisms underling neurogenic division of outer radial glial cells in the mammalian brain
We are interested in the function and regulation of neural stem cell in the mammalian brains. A unique population of neural stem cells, outer radial glial (oRG) cells, or basal radial glial (bRG) cells, residing in outer subventricular zone (OSVZ) of developing human neocortex has been recently characterized. These cells were believed to be responsible for the majority of cortical neuron production in human neocortex. However, little knowledge is known about how they contribute to the diversity of cell types found in the adult brain, due to the ethnic limitation in experimentally using human tissue.
We have developed fluorescence live imaging to recording the oRG cells division and lineage, and we have also established single cell sequencing techniques to identify the specific molecular related to oRG cells and the CRISPR-Cas9 techniques to generate conditional mutation in Ferret. With the combination technique of live imaging, genetics and bioinformatics approaches, we identified several genes specific expressed in oRG cells; we discovered the diverse lineages of oRG cells; and also we demonstrated that the ovexpression of oRG specifics genes enlarged the neocortex in mouse brain.
We hope our results will provide a solid new foundation for understanding the neocortical development with oRG cells involved, and shed lights on understanding human neurological diseases such as lissencephaly, autism, and schizophrenia.
Interests and expertise (Subject groups)