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Mechanics of development

Scientific meeting

Location

Kavli Royal Society Centre, Chicheley Hall, Newport Pagnell, Buckinghamshire, MK16 9JJ

Overview

Theo Murphy international scientific meeting organised by Dr Niamh Nowlan, Professor Celeste Nelson and Professor Philippa Francis-West.

Stain of actin and nuclei in an embryonic lung from a bearded dragon embryo. Image credit: Celeste Nelson, Princeton University

Mechanical forces are critical for embryonic development – yet very little is known about their roles. The meeting will focus on the role of mechanobiology in multiple aspects of embryonic development. The meeting will bring together multidisciplinary research groups in this exciting 'young' area of developmental biology for the first time, enabling formation of a network and sparking new collaborations.

The schedule of talks and speaker biographies can be found below. Recorded audio of the presentations will be available on this page after the meeting has taken place. Meeting papers will be published in a future volume of Philosophical Transactions B.

Poster session

There will be a poster session at 17.00 on Monday 5 February 2018. If you would like to apply to present a poster please submit your proposed title, abstract (not more than 200 words and in third person), author list, name of the proposed presenter and authors' institutions to the Scientific Programmes team. Please include the text ‘Poster abstract submission’  and the meeting title in the email subject line. Please note that places are limited and are selected at the scientific organisers' discretion. Poster abstracts will only be considered if the presenter is registered to attend the meeting.

Attending this event

This is a residential conference, which allows for increased discussion and networking.

  • Free to attend
  • Advanced registration essential (please request an invitation)
  • Catering and accommodation available to purchase during registration

Enquiries: contact the Scientific Programmes Team

Event organisers

Select an organiser for more information

Schedule of talks

05 February

Session 1 14:00-16:00

Developmental mechanics of branching and folding

2 talks Show detail Hide detail

Chairs

Professor Philippa Francis-West, King's College London, UK

Dr Niamh Nowlan, Imperial College London, UK

14:05-14:45 Active and passive forces in epithelial branching

Professor Celeste Nelson, Princeton University, USA

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14:45-15:30 Models and measurements of cortical folding

Dr Philip Bayly, University of Washington in St. Louis, USA

Abstract

Cortical folding, or gyrification, is critical to human brain development and function. The folded shape of the human brain allows the cerebral cortex, the thin outer layer of neurons and their associated processes, to attain a large surface area (~1600 cm2)  relative to brain volume (~1400 cm3). Abnormal cortical folding has been associated with severe cognitive and emotional disorders, including epilepsy, autism, and schizophrenia. Among different species the onset of cerebral cortical folding consistently takes place after the majority of cerebral cortical neurons have completed migration from their sites of origin (cell layers near the surface of the lateral ventricle) to the nascent cerebral cortex (the cortical plate). 

Despite decades of study the mechanical forces that lead to cortical folding remain incompletely understood. Leading hypotheses have focused on the roles of tangential growth, heterogeneities in the birth and migration of neurons, and internal tension in axons. Advances in the mathematical modelling of growth and morphogenesis, combined with new experimental data, promise to clarify the mechanical basis of cortical folding. Recent experimental studies have illuminated not only the fundamental cellular and molecular processes underlying cortical development, but also the stress state, mechanical properties, and spatiotemporal patterns of growth in the developing brain. The combination of mathematical modelling with these measurements allows us to evaluate hypothesized mechanisms objectively, and to ensure that they are consistent with physical law. Dr Bayly will review the neurobiology of cortical development, summarize pertinent experimental observations, and discuss the potential role of growth-induced instability in cortical folding.

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15:30-16:00 Coffee

Session 2 16:00-17:00

Keynote 1

1 talk Show detail Hide detail

Chairs

Dr Niamh Nowlan, Imperial College London, UK

16:00-17:00 From mechanobiology to developmentally-inspired engineering

Professor Donald Ingber, Wyss Institute at Harvard University, USA

Abstract

Professor Ingber will review work from his laboratory beginning with mechanistic studies relating to the biophysical basis of cell shape determination and tissue morphogenesis, and ending with demonstration of how the group has leveraged our understanding of the importance of mechanical forces as bioregulators to develop new medical devices and therapeutics. The work began with the hypothesis the mechanical forces are as important biological regulators as chemicals and genes, and that cells use tensegrity architecture to control their form and function. Testing this theory required development of new analytical tools, including magnetic cytometry, femtosecond laser nanosurgery, microcontact printing, and microfluidic culture systems. These studies led to confirmation of the critical role that physical forces play in developmental control, experimental confirmation of the cellular tensegrity model, and discovery that transmembrane integrins and cytoskeletal prestress mediate mechanotransduction and developmental control. Importantly, the group also leveraged these insights to develop new engineering innovations that are being translated into the clinic or commercial marketplace. Examples include: angiogenesis inhibitors for cancer therapy identified based on cell shape changes; ‘shrink wrap’-like polymer gels that trigger tissue and organ formation by physically inducing mesenchymal condensation; mechanotherapeutics that prevent pulmonary edema by inhibiting integrin-mediated mechanochemical signal transduction; shear stress-targeted drug delivery systems that are directed to sites of vascular occlusion and dissolve blood clots without systemic toxicity; and microfluidic cell culture devices lined by living human cells, known as ‘Organs-on-Chips’, which recapitulate organ-level structure and functions by providing appropriate mechanical cues as a way to replace animal testing for drug development, mechanistic discovery, and personalized medicine.

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17:00-18:30

Poster session

06 February

Session 3 08:30-11:00

Biomechanics of skeletal development

4 talks Show detail Hide detail

Chairs

Professor Andrew Pitsillides, The Royal Veterinary College, UK

Dr Chrissy Hammond, University of Bristol, UK

08:30-09:00 Uncovering the mechanistic basis of biomechanical input controlling skeletal development

Professor Paula Murphy, Trinity College Dublin, the University of Dublin, Ireland

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09:00-09:30 New players and concepts in muscoskeletal biomechanics

Professor Elazar Zelzer, Weizmann Institute of Science, Israel

Abstract

Muscle spindles and Golgi tendon organs (GTOs), proprioceptive mechanoreceptors located inside striated muscles and in myotendinous junctions, respectively, are components of the stretch reflex circuitry that control muscle activity. Using genetic mouse models, the group demonstrates the involvement of proprioception in regulating spine alignment and spontaneous realignment of fractured bones, dubbed natural reduction. Failure of the mechanism that maintains posture may result in spinal deformity as in adolescent idiopathic scoliosis. The group shows that null mutants for Runx3 transcription factor, which lack connectivity between proprioceptors and spinal cord, developed peripubertal scoliosis not preceded by vertebral dysplasia or muscle asymmetry. Deletion of Runx3 in the peripheral nervous system or specifically in peripheral sensory neurons, or of enhancer elements driving Runx3 expression in proprioceptive neurons, induced a similar phenotype. Egr3 knockout mice, lacking spindles but not GTOs, displayed a less severe phenotype, suggesting that both receptor types are required for this regulatory mechanism.

Fracture repair involves restoration of bone morphology. Comparison among mice of different ages revealed, surprisingly, that three-month-old mice exhibited more rapid and effective natural reduction than newborns. Fractured bones of Runx3-null mutants failed to realign properly. Blocking Runx3 expression in peripheral nervous system, but not in limb mesenchyme, recapitulated the null phenotype, as did inactivation of muscles flanking the fracture site. Egr3 knockout mice displayed a less severe phenotype, suggesting that both receptor types, as well as muscle contraction, are required for this regulatory mechanism. Overall, these findings uncover physiological roles for proprioception in non-autonomous regulation of skeletal integrity and repair. 

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09:30-10:00 The role of fetal movements in shaping the developing human skeleton

Dr Niamh Nowlan, Imperial College London, UK

Abstract

Mechanical stimulation generated by fetal kicking and movements is known to be important for prenatal musculoskeletal development, and there are a number of human conditions that emphasise the link between abnormal fetal movements and delayed or impaired skeletal development. The most common of these is developmental dysplasia of the hip (DDH), a relatively common joint shape abnormality (clinical incidence 1.3 in 1000), the risk of which is strongly associated with restricted fetal movement, such as fetal breech position. The group is using computational modelling approaches (finite element analysis and musculoskeletal modelling), combined with human fetal imaging data to try to understand how the biomechanical stimulation (stresses and strains) caused by fetal movements evolve in the prenatal developing hip joint. Furthermore, the group models a range of intra-uterine conditions and situations that increase the risk of DDH, such as fetal breech position and oligohydramnios (reduced amniotic fluid), in order to be able to understand how a range of factors affecting movement may impact on the developing hip joint.

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10:00-10:30 Talk title tbc

Professor Catherine K Kuo, University of Rochester, USA

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10:30-11:00 Coffee

Session 4 11:00-12:30

Developmental mechanics of tubes

2 talks Show detail Hide detail

Chairs

Professor Celeste Nelson, Princeton University, USA

Dr Chrissy Hammond, University of Bristol, UK

11:00-11:45 Notochord mechanics shape the vertebrate axis

Associate Professor Michel Bagnat, Duke University, USA

Abstract

The notochord is a conserved axial structure that serves as a hydrostatic scaffold for embryonic axis elongation and, later on, for proper spine assembly. The vertebrate notochord consists of a core of large vacuolated cells surrounded by an epithelial sheath that is encased by an elaborate extracellular matrix. Each vacuolated cell contains a single fluid filled vacuole that occupies most of the cellular volume. These cells are arranged in a stereotypical staircase pattern within the notochord. The group investigated the origin of this pattern and found that it can be achieved purely by physical principles. Moreover, the group is able to model the arrangement of vacuolated cells within the notochord using a physical model composed of silicone tubes and water absorbing beads, suggesting that the notochord is a self-organizing structure. Furthermore, it was found that the biological structure and the physical model can be accurately described by the theory developed for cylindrical foams. 

In spite of their large size and being under constant mechanical stress, vacuolated cells possess a remarkable structural integrity. This is in part due to the presence of numerous caveolae, omega shaped cell surface invaginations, at the plasma membrane of vacuolated cells, which buffer mechanical tension. In zebrafish that lack caveolae, vacuolated cells collapse under the strain of axial bending during locomotion. Then, release of nucleotides from collapsed vacuolated cell leads to the invasion and trans-differentiation of sheath cells into new vacuolated cells. This regenerative response restores the mechanical properties of the notochord, thus allowing normal spine development.

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11:45-12:30 Talk title tbc

Professor Mark Kahn, University of Pennsylvania, USA

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12:30-13:30

Lunch

Session 5 13:30-15:30

Signalling and mechanotransduction during cardiovascular development

2 talks Show detail Hide detail

Chairs

Professor Philippa Francis-West, King's College London, UK

Professor Andrew Pitsillides, The Royal Veterinary College, UK

13:30-14:15 A mechanical coupling checkpoint required for mechanotransduction in endothelial cells

Professor Ellie Tzima, University of Oxford, UK

Abstract

The Tzima lab investigates the role of mechanotransduction in regulating cardiovascular function in health and disease. We focus on endothelial mechanosensing and how fluid shear stress affects vessel and cardiac morphogenesis and function. Over the last decade, we identified and systematically characterised one of the most comprehensive models of endothelial mechanotransduction available to date. Using state-of-the-art approaches we showed that PECAM is a part of a junctional mechanosensory complex that senses and transduces mechanical force that ultimately regulates flow-mediated vascular remodelling. PECAM does not act alone; it crosstalks with integrins, another established mechanotransduction site, to collectively determine downstream responses. Recent work from our group, identified the signalling adaptor protein Src homologous and collagen protein (Shc) as a major player in endothelial mechanotransduction. We showed that Shc is tyrosine phosphorylated in response to mechanical force and binds to both the junctional mechanosensory complex and integrins. We now present data showing that Shc tyrosine phosphorylation provides a mechanical coupling checkpoint that allows certain signals to proceed, while blocking others in endothelial cells.

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14:15-15:00 Tissue mechanics and mechanical forces during cardiovascular development

Dr Julien Vermot, Institut de génétique et de biologie moléculaire et cellulaire, France

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15:00-15:30 Coffee

Session 6 15:30-17:00

Morphodynamics and hemodynamics

2 talks Show detail Hide detail

Chairs

Professor Celeste Nelson, Princeton University, USA

Dr Nic Tapon, The Francis Crick Institute, UK

15:30-16:15 Tissue dynamics in development and repair

Dr Yanlan Mao, University College London, UK

Abstract

Actomyosin contractility is a key regulator of tissue dynamics. During development, tissue dynamics, such as cell intercalations and oriented cell divisions, are critical for shaping tissues and organs. However, less is known about how tissues regulate their dynamics during tissue homeostasis and repair, to maintain their shape after development. In this talk, Dr Mao will discuss how tissues respond to mechanical perturbations, such as stretching or wounding, by altering their actomyosin contractile structures, to change tissue dynamics, and thus preserve tissue shape and patterning.

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16:15-17:00 Blood flow and heart formation

Professor Sandra Rugonyi, Oregon Health & Science University, USA

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07 February

Session 7 09:00-11:00

Signalling, self-organisation and pattern formation

2 talks Show detail Hide detail

Chairs

Professor Philippa Francis-West, King's College London, UK

Dr Nic Tapon, The Francis Crick Institute, UK

09:00-09:45 Mechanical control of intestinal organoid formation

Professor Matthias Lutolf, École polytechnique fédérale de Lausanne, Switzerland

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09:45-10:30 Physical organogenesis of the gut

Dr Nicolas R Chevalier, Laboratoire Matière et Systèmes Complexes; CNRS - Université Paris Diderot, France

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10:30-11:00 Coffee

Session 8 11:00-12:30

Keynote 2, summary and close

2 talks Show detail Hide detail

Chairs

Professor Philippa Francis-West, King's College London, UK

11:00-12:00 Mechanbiology of mammalian epidermis

Professor Fiona Watt FMedSci FRS, King's College London, UK

Abstract

The ability to reconstitute human epidermis in culture has afforded the opportunity to examine stem cell-niche interactions at single cell resolution. Professor Watt will describe the responses of human epidermal stem cells to different topographical cues, ranging from the 1 to 100 micron scale. Professor Watt will describe the signal transduction pathways that lead to the onset of terminal differentiation and show how different cues trigger differentiation via different pathways.

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12:00-12:30 Summary of discussions and closing remarks

12:30-14:00

Lunch

Mechanics of development Kavli Royal Society Centre, Chicheley Hall Newport Pagnell Buckinghamshire MK16 9JJ