Research Fellows Directory
Dr Michal Malecki
University College London
My research is focused on the regulation of transcription and changes in transcript stability in response to environmental stimuli. One of the aims of my project is to investigate the role of cytoplasmic non-canonical RNA polymerases in controlling the fate of posttranscriptional RNA. To do this, I am using the model organism Schizosaccharomyces pombe. My main research focus will be on the role of RNA uridylation, one of the modifications catalysed by non-canonical polymerases. Recently, uridylation was shown to be widespread across different species, and a function in RNA destabilisation was suggested. However, there is still no consensus about the function, importance and exact mechanism of this process. I believe that research on S. pombe using high throughput approaches like RNA sequencing may help to develop our knowledge about the function of non-canonical polymerases.
I am also interested in the transcriptional changes S. pombe undergoes in fermentation and respiration. Fission yeast is a facultative aerobe, and in contrast to budding yeast, requires mitochondria. Investigating transcriptional changes in respiratory and fermentative media provides a good model to study gene expression regulation. Moreover, cellular metabolism in investigated conditions is universal, and comparisons can easily be drawn with the metabolic states of mammalian cells. For example, cancer cells preferentially use aerobic fermentation over respiration for energy production.
Interests and expertise (Subject groups)